1.学校一般有自己的ATCC帐号，如果不知道有没有可以打电话问中原公司他们可以查到。2.下载客户订单表（在中原公司官网里面能找到）按照上面说的填写就好了，然后发给公司联系人，她会给你一份合同。要签字盖章后，扫描或者传真给她。3.中原公司收到订单全款后每周五给ATCC那边下单，有货的话4-6周就能收到了。4.购买不满1700元要补运费500元。
人肺细胞株
CCD-19Lu
Organism： Homo sapiens, human Tissue: lung Cell Type: fibroblast Product Format： frozen Morphology： fibroblast Culture Properties：adherent Disease：normal Age：20 years
Gender: female
Ethnicity ：Caucasian白人
Storage Conditions： liquid nitrogen vapor phase Karyotype： normal female; diploid; stable
Derivation: The line was derived from normal tissue from
patient who died of trauma to the head.
Clinical Data :20 years ，Caucasian，female
Comments：The cells are capable of at least 8 or 9 population doublings
beyond the current freeze.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% Subculturing：Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor(胰蛋白酶抑制剂）.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation：Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions: Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
CCD-34Lu
Organism： Homo sapiens, human Tissue: lung Cell Type: fibroblast Product Format： frozen Morphology： fibroblast Culture Properties：adherent Disease：normal Age：2.5weeks Gender: female Ethnicity ：black
Derivation： The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%
Subculturing：Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
CCD-16Lu
Organism： Homo sapiens, human Tissue: lung Cell Type: fibroblast Product Format： frozen
Morphology： fibroblast Culture Properties：adherent Disease：normal Age：35years Gender: male Ethnicity ：Caucasian
Karyotype: Normal human male; diploid stable
Derivation: The line was derived from normal tissue from a patient who died of a class 1 - 2 astrocytoma.
Comments:This cell line undergoes senescence at a Population Doubling Level (PDL) of about 29.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Volumes used in this protocol are for 75 cm2 flask;
proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #4301) are recommended for subculturing this product.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
4. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
5. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
6. Add appropriate aliquots of the cell suspension to new culture vessels.
7. Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
CCD 18Lu
Organism： Homo sapiens, human Tissue: lung Cell Type: fibroblast Product Format： frozen Morphology： fibroblast Culture Properties：adherent Disease：normal Age：2.5 months Gender: male Ethnicity ：BLACK
Karyotype: modal number = 46; range = 35 to 47
The stemline number is diploid and stable. The karyotype is that of a human female
Derivation: This fibroblast-like cell line was derived from the lung tissue of a 2 month, 17-day-old Black female
Comments:This fibroblast-like cell line was derived from the lung tissue of a 2 month, 17-day-old Black female. The donor had cerebral anoxia, cardiac anomaly, sepsis, endocardial cushion defect and fetal alcoholic syndrome. The line was initiated in January, 1979 at the American Type Culture Collection from enzyme-dispersed tissue. The cells senesce at about population doubling level (PDL) 60 to 70.
CCD-11Lu
Derivation :The line was derived from normal tissue from patient who died following trauma to the head.
Antigen Expression: blood type O; Rh +
Comments:The cells are capable of at least 12 population doublings beyond the current freeze.
Age：9 years Gender: male Ethnicity ：Asian
CCD-33Lu
Age：7 years Gender: male Ethnicity ：Caucasian
Derivation:The line was derived from normal lung tissue from a child who died of a brain tumor.
