Inhibitors, Agonists, Screening Libraries
www.MedChemExpress.com
Data Sheet
Product Name:Cat. No.:CAS No.:
Molecular Formula:Molecular Weight:Target:Pathway:Solubility:
HOE 32020HY-1562923554-99-6C25H23ClN42.94DNA Stain
Cell Cycle/DNA DamageDMSO: ≥ 69 mg/mL
BIOLOGICAL ACTIVITY:
HOE 32020 is a Hoechst stain, which is a blue fluorescent dyes used to stain DNA.IC50 Value:
These Bis–benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emissionspectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemissionmaximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510–540 nm range. Hoechst dyes are soluble inwater and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL.Aqueous solutions are stable at 2–6 °C for at least six months when protected from light. For long–term storage the solutions areinstead frozen at ≤–20 °C.
The dyes bind to the minor groove of double–stranded DNA with a preference for sequences rich in adenine andthymine. Althoughthe dyes can bind to all nucleic acids, AT–rich double–stranded DNA strands enhance fluorescence considerably.
Hoechst dyes are cell–permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, whichmeans that cells survive a treatment with these compounds. Cells that express specific ATP–binding cassette transporter proteins canalso actively transport these stains out of their cytoplasm.
References:
[1]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal ofhistochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493–505.[2]. a b c \"Hoechst Stains\". Invitrogren (Molecular Probes).
[3]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6–diamidine–2–phenylindole and bisbenzimide (Hoechst 33258). A comparativefootprinting study. Biochimica et Biophysica Acta 949 (2): 158–68.
Caution: Product has not been fully validated for medical applications. For research use only.
Tel: 609-228-68 Fax: 609-228-5909 E-mail: tech@MedChemExpress.com
Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA
www.MedChemExpress.com
