专利内容由知识产权出版社提供
专利名称：Methods for delivering biologically active
molecules into cells
发明人：Jeffery L. Miller,Urszula Wojda,Paul K.
Goldsmith
申请号：US09483434申请日：20000114公开号：US06974698B1公开日：20051213
摘要：Methods for delivering a biologically active molecule into a cell by linking amolecule to the cell surface, wherein the molecule can act as a surface receptor, thencomplexing the biologically active molecule with a ligand for the surface receptor, andfinally contacting the biologically active molecule-ligand complex with the cell surfaceare disclosed. Delivery of any biologically active molecule, e.g. proteins, enzymes, nucleicacids, hormones, nucleic acids, and oligonucleotides, is contemplated. The use of biotin orbiotinylated antibodies as the surface receptor is disclosed. The use of PEI and PEI-avidinconjugates complexed with oligonucleotides for delivery into a directly or indirectlybiotinylated cell surface, along with the PEI-avidin-nucleic acid compositions, are
disclosed. Primary and cultured cells with a covalently linked surface receptor molecule,such as biotin, on their surfaces are also disclosed.
申请人：Jeffery L. Miller,Urszula Wojda,Paul K. Goldsmith
地址：Potomac MD US,Warsaw PL,Rockville MD US
国籍：US,PL,US
代理机构：Needle & Rosenberg, PC
更多信息请下载全文后查看
