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专利名称：NOVEL METHODS FOR THE
CHARACTERIZATION OF COMPOUNDSWHICH STIMULATE STF-1 EXPRESSION INPANCREATIC ISLET CELLS
发明人：MONTMINY, Marc, R.,SHARMA, Seema申请号：EP97903918.7申请日：19970122公开号：EP08792B1公开日：20050907
摘要：One embodiment of the present invention provides a method of testing forcompounds which may induce STF-1 transcription. Pancreatic islet cells which express theSTF-1/lacz fusion gene are isolated and the effect of various compounds on STF-1expression is measured by adding the compound of interest to STF-1/lacZ expressingcells. LacZ activity in control and treated cells is then quantitated by a colorimetric assay.Using this method, a large number of compounds can be screened and STF-1 inducingcompounds can be identified readily. Another embodiment of the present inventionprovides a method of using the STF-1 promoter to mark insulin producing pancreatic isletcells in vivo. In this regard, the green fluorescent protein (GFP) served as an indicator. Theintroduction of the STF-1 green fluorescent protein transgene into pigs allows for theefficient and rapid recovery of insulin producing cells from the pancreas.
申请人：RES DEV FOUNDATION
地址：US
国籍：US
代理机构：McQueen, Andrew Peter
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