USP关于色谱柱填料的规定

L1和L8是美国药典(USP)规定的色谱柱编号，其实就是C18柱和NH2柱。下面是对应的色谱柱类型。

L1：十八烷基键合多孔硅胶或无机氧化物微粒固定相， 简称C18或ODS L2：30～50um表面多孔薄壳型键合C18(ODS)固定相 L3：多孔硅胶微粒 即一般的硅胶柱 L4：30～50um表面多孔薄壳型硅胶 L5：30～50um表面多孔薄壳型氧化铝

L6：30～50um实心微球表面包覆磺化碳氟聚合物－强阳离子交换固定相 L7：全多孔硅胶微粒键合C8官能团固定相 简称C8柱 L8：全多孔硅胶微粒键合非交联NH2固定相 简称NH2柱 L9：强酸性阳离子交换基团键合全多孔不规则形硅胶固定相 L10：多孔硅胶微球键合氰基固定相（CN） 简称CN柱 L11：键合苯基多孔硅胶微球固定相 简称苯基柱 L12：无孔微球键合季胺功能团的强阴离子填料

L13：三乙基硅烷化学键合全多孔硅胶微球固定相（C1） 简称C1柱 L14:10um硅胶化学键合强碱性季铵盐阴离子交换固定相 简称SAX柱 L15：已基硅烷化学键合全多孔硅胶微球固定相 简称C6柱 L16：二甲基硅烷化学键合全多孔硅胶微粒固定相

L17：氢型磺化交联苯乙烯-二乙烯基苯共聚物,强阳离子交换树脂 L18: 3～10um全多孔硅胶化学键合胺基（NH2）和氰基（CN） L19：钙型磺化交联苯乙烯－二乙烯基苯共聚物，强阳离子交换树脂 L20：二羟基丙烷基化学键合多孔硅胶微球固定相（Diol） 简称二醇基柱 L21：刚性苯乙烯－二乙烯基苯共聚物微球 L22：带有磺酸基团的多孔苯乙烯阳离子交换树脂

L23：带有季胺基团的聚甲基丙烯酸甲酯或聚丙烯酸酯多孔离子交换树脂 L24：表面含有大量羟基的半刚性聚乙烯醇亲水凝胶

L25：聚甲基丙烯酸酯树脂交联羟基醚（表面含有残余羧基功能团）树脂。能分离分子量100～5000MW范围的水溶性中性、阳离子型及阴离子型聚合物（用聚氧乙烯测定）的固定相

L26：丁基硅烷化学键合全多孔硅胶微球固定相 L27：30～50um的全多孔硅胶微粒

L28：多功能载体，100?的高纯硅胶加以氨基键合以及C8反相键合的官能团 L29: 氧化铝,反相键合,含碳量低,氧化铝基聚丁二稀小球,5um，孔径80? L30: 全多孔硅胶键合乙基硅烷固定相

L31: 季胺基改性孔径2000?的交联苯乙烯和二乙烯基苯(55%)强阴离子交换树脂 L32: L-脯氨酸铜配合物共价键合于不规则形硅胶微粒的配位体的交换手性色谱填料 L33: 能够分离分子量4000～40000MW范围蛋白质分子的球形硅胶固定相,pH稳定性好 L34：铅型磺化交联苯乙烯－二乙烯基苯共聚物强阳离子交换树脂，9um球形 L35：锆稳定的硅胶微球键合二醇基亲水分子单层固定相，孔径150? L36: 5um胺丙基硅胶键合L-苯基氨基乙酸－3,5二硝基苯甲酰 L37：适合分离分子量2000～40,000Mw的聚甲基丙烯酸酯凝胶 L38：水溶性甲基丙烯酸酯基质SEC色谱柱 L39：亲水全多孔聚羟基甲基丙烯酸酯色谱柱

L40：Tris 3,5－二甲基苯基氨基甲酸酯纤维素涂覆多孔硅胶微球 L41：球形硅胶表面固定α1酸糖蛋白固定相 L42: C8和C18硅烷化学键合多孔硅胶固定相 L43: 硅胶微球键合五氟代苯基固定相

L44: 多功能固定相,60 ?高纯硅胶基质键合磺酸阳离子交换功能团和C8反相功能团 L45: β-环糊精键合多孔硅胶微球

L46: 季胺基改性苯乙烯-二乙烯基苯聚合物微球

液相色谱柱

USP column Packing

L1 —— Octadecyl silane chemically bonded to porous silica or ceramic micro-particles,3 to 10um in diameter

L2 —— Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50um in diameter.

L3 —— Porous silica particles, 5 to 10um in diameter.

L4 —— Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50um in diameter.

L5 —— Alumina of controlled surface porosity bonded to a solid spherical core , 30 to 50um in diameter.

L6 —— Strong cation –exchange packing-sulfonated fluorocarbon polymer coated on a solid spherical, 30 to 50um in diameter. L7 —— Octylsilane chemically bonded to totally porous silica particles , 3 to 10um in diameter.

L8 —— An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 10um in diameter.

L9 —— 10um irregular or spherical layer of aminopropylsilane chemically bonded, strongly acidic cation-exchange coating. L10- Nitrile groups chemically bonded to porous silica particles, 3 to 10um in diameter.

L11- Phenyl group chemically bonded to porous silica particles, 5 to 10um in diameter.

L12- A strong anion-exchange packing made by chemically bonding a quaternary ammonium anion-exchange coating. L13- Trimethylsilane chemically bonded to porous silica particles, 3 to 10um in diameter.

L14 – Silica gel 10um in diameter having a chemically bonding a quaternary ammonium anion-exchange coating.

L15 – Hexylsilane chemically bonded to totally porous silica particles, 3 to 10um in diameter.

L16 – Dimethylsilane chemically bonded to porous silica particles, 5 to 10um in diameter.

L17_ Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen from, 7 to 11um in diameter.

L18 – Amion and cyano groups chemically bonded to porous silica particles, 3 to 10um in diameter.

L19 – Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divilbenzene copolymer in the calcaium from, about 10um in diameter.

L20- Dihydroxypropane groups chemically bonded porous silica particles , 5 to 10um in diameter.

L21 – A rigid, spherical styrene-divinylbenxene copolymer, 5 to 10um in diameter.

L22 – A cation-exchange resin made of porous polystyrene gel with sulfonic acid group, about 10um in diameter.

L23 – An cation-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, about 10um in size.

L24 — A semi-right hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix surface , 32 to 63um in diameter.5

L25 _Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applied to neutral, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with poly-hydroxylated ether (surface contained some residual carboxylfunctional groups) was fund suitable.

L26- Butyl silica chemically bonded to totally porous silica particles, 5 to 10um in diameter.

L27 _ Porous silica particles, 30 to 5um in diameter.

L28 – A multifunctional support, which consists of a high purity, 100 Å, spherical silica substrate that has been bonded with anionic exchange, amine functionality in addition to a conventional reversed phase C8 functionality.

L29_ Gamma alumina, reverse-phase, low carbon percentage by weight, alumina-based polybutadiene, spherical particles. 5um in diameter with a pore volume of 80 Å

L30- Ethyl silica chemically bonded to totally porous silica particles, 3 to 10um in diameter.

L31 – A strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5um macroporous particles having a pore size of 2000Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene.

L32 – A chiral ligand-exchange packing-L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10um in diameter.

L33- Packing having the capacity to separate dextrans by molecular size over a range of 4000 to 500000 Da. It is spherical, silica-based, and processed to provide PH stability.6 L34 – Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinybenzene copolymer in the lead form, about 9um in diameter.

L35- A zirconium-stabilized spherical silica packing with a

hydrophilic(diol-type) molecular monolayer bonded phase having p pore size of 150Å.

L36 – A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5um aminopropyl silica.

L37 – Packing having the capacity to separate proteins by molecular size over range of 2000 to 40000Da. It is a polymethacrylate gel.

L38 – A methacrylate-based size-exclusion packing for water-soluble samples.

L39- A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.

L40 – Cellulose tris-3,5-dimethylphenylcarbmate coated porous silica particles, 5 to 20umin diameter.

L40—Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles,5 to 20 μm indiameter.

L41－Immobilized а1 acid glycoprotein on spherical silica particles,5μm in diameter.

L42—Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5μm in diameter.

L43—Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to 10μm in diameter. L44—A multifunctional support,which consists of a high

purity,60Å,spherical silica substrate that has been bonded with a cationic exchanger,sulfonic acid functionality

in addition to conventional reversed phase C8 functionality. L45—Beta cyclodextrin bonded to porous silica particles, 5 to 10μm in diameter.

L46—Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, 10μm in diameter. L47—High-capacity anion-exchange microporous substrate,funlly functionalized with trimethlyamine groups,8μm in diameter.7 L48－Sulfonated,cross-linked polystyrene with an outer layer of submicron,porous,

anion-exchange microbends 15μm in diameter.

L49－A reversed-phase packing made by coating a thin layer of polybutadiene onto spherical porous zirconia particles,3 to 10μm in diameter.8

L50－Multifunction resin with reversed-phase retention and strong anion-exchange functionalities.The resin consists of ethylvinylbenzene 55％cross-linked with divinylbenzene

copolymer,3 to 15μm in diameter,and a surface area not less than 350㎡per g.Substrate is coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene.9

L51—Amylose

tris-3,5-dimethylphenylcarbamate-coated,porous,spherical,silica particles, 5 to 10μm in diameter.10

L52—A strong cation exchange resin made of porous silica with sulfopropyl groups, 5 to 10μm in diameter.11

L53—Weak cation-exchange consisting of ethylvinylbenzene,55％cross-linked with divinylbenzene copolymer, 3 to 15μm in diameter.Substrate is surface grafted with carboxylic acid and/orphosphoric acid functionalized monomers.Capacity not less than 500μEq/column.12

L54－A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads,about 13 μm in diameter.13

L55－A strong cation-exchange resin made of porous silica coated with polybutadiene-maleic acid copolmer,about 5 μm in diameter.14

L56－Isopropyl silane chemically bonded to totally porous silica paryicles,3 to 10μm in diameter.15

L57－A chiral recognition protein,ovomucoid,chemically onded to silica particles,about 5μm in diameter,with a pore size of 120Å

L58－Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form,about7to 11μm in diameter,16

▲ L59－Packing having the capoacity to separate proteins bymolecular weight over the range of 10 to 500kDa.It is

spherical(10μm),silica-based, and processed to provide hydrophilic characteristics and pH stability.17▲USP28

▲ L60－Spherical,porous silica gel,3 or 5μm in diameter,the surface of which has been covalently modified with

palmitamido-propyl groups and endcapped with acetamidopropyl groups to a ligand density of about 6 μmoles per ㎡.18▲USP28

L61 A hydroxide selective strong anion-exchange resin consisting of a highly cross-linked core of 13 µm microporous particles having a pore size less than 10 Angstrom units and consisting of ethylvinylbenzene cross-linked with 55%

divinylbenzene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quartenary ammonium ions (6%) L62 C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in diameter

L63 Glycopeptide teicoplanin linked through multiple covalent bonds to a 100 Angstrom units spherical silica.

L Strongly basic anion exchange resin consisting of 8% crosslinked styrene divinylbenzene copolymer with a quartenary ammonium group in the chloride form, 45 to 180 µm in diameter. L65 Strongly acidic cation exchange resin consisting of 8% sulfonated crosslinked styrene divinylbenzene copolymer with a sulfonic acid group in the hydrogen form, 63 to 250 µm in diameter. L66 A crown ether coated on a 5 µm particle size silica gel substrate. The active site is (S)-18-crown-6-ether.

L67 Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer.

L68 Spherical, porous silica, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not endcapped.

L69 Ethylvinylbenzene/divinylbenzene substrate agglomerated with quartenary amine functionalized 130 nm latex beads, about 6.5 µm in diameter.

L70 Cellulose tris(phenyl carbamate) coated on 5 µm silica L71 A rigid, spherical, polymethacrylate, 4 to 6 µm in diameter L72 (S)-phenylglycine and 3,5-dinitroanaline urea linkage covalently bonded to silica.

L73 A rigid spherical polydivinylbenzene particle, 5 to 10 µm in diameter

L74 A strong anion-exchange resin consisting of a highly cross-linked core of 7 µm macroporous particles having a 100 Angstroms average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion-exchange layer grafted to the surface, which is functionalized with alkyl quartenary ammonium ions.

气相色谱柱 Phases

G1－Dimethylpolysilxane oil. G2－Dimethylpolysilxane gum.

G3－50%Phenyl-50% methylpolysiloxane. G4－Diethylene glycol succinate polyester. G5－3-Cyanopropylpolysiloxane. G6－Trifluoropropylmethylpolysiloxane.

G7－50%3-Cyanopropyl-50%phenylmethylsilicone. G8－80% Bis(3-cyanopropyl)-20%

3-cyanopropylphenylpolysi-loxane(percentages refer to molar substitution).

G9－Methylvinylpolysiloxane.

G10－Polyamide formed by reacting a C36 dicarboxylic acid with 1,3-di-4-piperidylpropane and piperidine in the respective mole ratios of 1.00:0.90:0.20.

G11－Bis(2-ethylhexyl)sebacate polyester. G12－Phenyldiethanoamine succinate polyester. G13－Sorbitol.

G14－Polyethylene glycol(av.mol.wt.of 950 to 1050). G15－Polyethylene glycol(av.mol.wt.of 3000 to 3700).

G16－Polyethylene glycol compound(av.mol.wt.about 15,000).A high molecular weight compound of polyethene glycol with a diepoxide linker.Available commercially as Polyethylene Glycol Compound 20M,or as Carbowax 20M,from suppliers of chromatographic reagents.

G17－75% Phenyl-25% methylpolysiloxane. G18－Polyalkylene glycol.

G19－25% Phenyl-25% cyanopropyl-50% methylsilicone. G20－Polyethylene glycol(av.mol.wt.of 380 to 420). G21－Neopentyl glycol succinate. G22－Bis(2-ethylhexyl)phthalate. G23－Polyethyl glycol adipate. G24－Diisodecyl phthalate.

G25－Polyethylene glycol compound TPA.A high molecular weight compound of a polyethylene glycol and a diepoxide as Carbowax 20M-TPA from suppliers of chromatographic reagents. G26－25% 2-Cyanoethyl-75% methylpolysiloxane. G27－5% Phenyl-95% methylpolysiloxane. G28－25% Phenyl-75% methylpolysiloxane. G29－3,3’-Thiodipropionitrile.

G30－Tetraethylene glycol dimethyl ether.

G31－Nonylphenoxypoly(ethyleneoxy)ethanol (av.ethyleneoxy chain length is 30);Nonoxynol 30.

G32－20% Phenylmethyl-80% dimethylpolysiloxane. G33－20% Carborane-80% methylsilicone.

G34－Diethylene glycol succinate polyester stabilized with phosphoric acid.

G35－A high molecular weight compound of a polyethylene glycol and a diepoxide that is esterified with nitroterephthalic acid. G36－1% Vinyl-5% phenylmethylpolysiloxane. G37－Polyimide.

G38－Phase G1 containing a small percentage of a tailing inhibitor.19

G39－Polyethylene glycol(av.mol.wt.about 1500). G40－Ethylene glycol adipate.

G41－Phenylmethyldimethylsilicone(10% phenyl-substituted). G42－35% phenyl-65% dimethylpolysiloxane(percentages refer to molar substitution).

G43－6% cyanopropyhenyl-94%

dimethylpolysiloxane(percentages refer to molar substitution). G44－2% low molecular weight petrolatum hydrocarbon grease and 1% solution of potassium hydroxide.

G45－Divinylbenzene-ethylene glycol-dimethylacrylate. G46－14% Cyanopropylphenyl-86% methylpolysiloxane. G47－Polvethylene glycol(av.mol.wt.of about 8000). G48－Highly polar,partially cross-linked cyanopolysiloxane. G49－Proprietary derivatized phenyl groups on a polysiloxane backbone.20 Supports

NOTE－Unless otherwise specified,mesh sizes of 80 to 100 or,alternatively,100 to 120 are intended.

S1A－Siliceous earth for gas chromatography has been fluxcalcined by mixing diatomite with Na2CO3 flux and calcining above 900°.The siliceous earth is acid-washed ,then water-washed until neutral,but not base-washed.The silieous earth may be silanized by treating with an agent such as

dimethyldichlorosilane21 to mask surface silanol groups.

S1AB－The siliceous earth as described above is both acid-and base-washed.21

S1C－A support prepared from crushed firebrick and calcined or burned with a clay binder above 900°with subsequent acid-wash.It may be silanized.

S1NS－The siliceous earth is untreated.

S2－Styrene-divinylbenzene copolymer having a nominal surface area of less than

50㎡ per g and an average pore diameter of 0.3 to 0.4 μm. S3－Copolymer of ethylvinylbenzene and divinylbenzene having a nominal surface ared of 500 to 600 ㎡per g and an average pore diameter of 0.0075μm.

S4－Styrene-divinylbenzene copolymer with aromatic-O and –N groups, having a nominal surface area of 400 to 600㎡ per g and an average pore diameter of 0.0076μm. S5－40-to60-mesh,high-molecular weight tetrafiuorethylenepolymer.

S6－Styrene-divinylbenzene copolymer having a nominal surfasearea of 250 to 350㎡ per g and an average pore diameter of 0.0091μm.

S7-Graphitized carbon having a nominal surface area of 12㎡ per g.

S8-Copolymer of 4-vinyl-pyridine and styrene-divinylbenzene. S9-A porous polymer based on 2,6-diphenly-p-phenylene oxide. S10-A highly polar cross-linked copolymer of acrylonitrite and divinylbenzene.

S11-Graphitized canbon having a nominal surface area of 100㎡ per g modified with small amounts of petrolatum and polyethylene glycol compound,22

S12-Graphitized carbon having a nominal surface ared of 100 ㎡ per g.