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Electrophoresis system in series for multiple agar

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专利内容由知识产权出版社提供

专利名称:Electrophoresis system in series for multiple

agarose slab gels

发明人:Hamelin, Claude申请号:EP85109369.0申请日:19850725公开号:EP0173081A1公开日:19860305

专利附图:

摘要:The disclosure describes an apparatus for the electrophoresis in series ofmultiple agarose slab gels. It comprises a train of at least three elongated box-likecontainers (1, 2, ... 12) such as twelve, which are set consecutively in substantially parallel

fashion at a variable distance from one another, each container to contain a runningbuffer (21, 22, 23, 24). The first and last containers (1, 12) are each provided with anelectrode (17,19) for connection to a single suitable power supply. A plurality of

horizontal plates (31, 33), each to contain a slab gel (35), are disposed in bridging fashionover adjacent spaced apart containers, to cover gaps between all the containers (1, 2,...12). The apparatus comprises first means (39) capable of establishing capillary contactbetween one end of the slab gel (35) and the running buffer which is present in a givencontainer; and second means (39) capable of establishing capillary contact between theother end of the slab gel (35) and the running buffer which is present in the nextcontainer. A method for the separation of components from a mixture of molecules ofmixed molecular weights, wherein a solution of said molecules is added to wells (37)formed in a horizontal slab gel (35) and the gel is thereafter treated by the aboveelectrophoresis to produce localized bands each being characteristic of a specificmolecular weight, is also disclosed.

申请人:INSTITUT ARMAND-FRAPPIER

地址:531 Boulevard des Prairies Laval Quebec H7N 4Z3 CA

国籍:CA

代理机构:Patentanwälte Ruff, Beier, Schöndorf und Mütschele

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