专利名称:Electrophoresis system in series for multiple
agarose slab gels
发明人:Hamelin, Claude申请号:EP85109369.0申请日:19850725公开号:EP0173081A1公开日:19860305
专利附图:
摘要:The disclosure describes an apparatus for the electrophoresis in series ofmultiple agarose slab gels. It comprises a train of at least three elongated box-likecontainers (1, 2, ... 12) such as twelve, which are set consecutively in substantially parallel
fashion at a variable distance from one another, each container to contain a runningbuffer (21, 22, 23, 24). The first and last containers (1, 12) are each provided with anelectrode (17,19) for connection to a single suitable power supply. A plurality of
horizontal plates (31, 33), each to contain a slab gel (35), are disposed in bridging fashionover adjacent spaced apart containers, to cover gaps between all the containers (1, 2,...12). The apparatus comprises first means (39) capable of establishing capillary contactbetween one end of the slab gel (35) and the running buffer which is present in a givencontainer; and second means (39) capable of establishing capillary contact between theother end of the slab gel (35) and the running buffer which is present in the nextcontainer. A method for the separation of components from a mixture of molecules ofmixed molecular weights, wherein a solution of said molecules is added to wells (37)formed in a horizontal slab gel (35) and the gel is thereafter treated by the aboveelectrophoresis to produce localized bands each being characteristic of a specificmolecular weight, is also disclosed.
申请人:INSTITUT ARMAND-FRAPPIER
地址:531 Boulevard des Prairies Laval Quebec H7N 4Z3 CA
国籍:CA
代理机构:Patentanwälte Ruff, Beier, Schöndorf und Mütschele
更多信息请下载全文后查看
因篇幅问题不能全部显示,请点此查看更多更全内容