Multifunctional alleles

专利名称：Multifunctional alleles

发明人：Economides, Aris N.,Murphy, Andrew

J.,Lengyel, Peter Matthew,Yang, Peter H. A.

申请号：AU2010319894申请日：20101029

公开号：AU2010319894B2公开日：20150305

摘要：Nucleic acid constructs and methods for rendering modifications to a genomeare provided, wherein the modifications comprise null alleles, conditional alleles and nullalleles comprising COINs. Multifunctional alleles (MFA) are provided, as well as methodsfor making them, which afford the ability in a single targeting to introduce an allele thatcan be used to generate a null allele, a conditional allele, or an allele that is a null alleleand that further includes a COIN. MFAs comprise pairs of cognate recombinaserecognition sites, an actuating sequence and/or a drug selection cassette, and anucleotide sequence of interest, and a COIN, wherein upon action of a recombinase aconditional allele with a COIN is formed. In a further embodiment, action of a secondrecombinase forms an allele that contains only a COIN in sense orientation. In a furtherembodiment, action by a third recombinase forms an allele that contains only theactuating sequence in sense orientation.

申请人：Regeneron Pharmaceuticals, Inc.

代理人：Phillips Ormonde Fitzpatrick

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