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Quantifying the Local Reliability of a Sequence Alignment

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insect globin / myoglobin

0.20.180.16fraction of residue pairs in alignment0.140.120.10.080.060.040.0200

0.10.20.30.4

0.5reliability

0.60.70.80.91

homology class 25 % − 30 %

0.2

fraction of residue pairs0.15

0.1

0.05

00

1234

567reliability index x 10

10

homology class 30 % − 40 %0.2fraction of residue pairs0.150.10.05001234567reliability index x 1010homology class 40 % − 50 %0.2fraction of residue pairs0.150.10.05001234567reliability index x 1010homology class 25 % − 30 %

1mean and standard deviation of error0.8

0.6

0.4

0.2

0

−0.2

0

123

456reliability index x 10homology class 30 % − 40 %710

1mean and standard deviation of error0.80.60.40.20−0.20123456reliability index x 10homology class 40 % − 50 %7101mean and standard deviation of error0.80.60.40.20−0.20123456reliability index x 10710

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